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- Title
Intercellular trafficking of VP22-GFP fusion proteins is not observed in cultured mammalian cells.
- Authors
Fang, B; Xu, B; Koch, P; Roth, J A
- Abstract
Herpes simplex virus type 1 (HSV-1) VP22 was recently reported to mediate intercellular trafficking of a protein fused to the C-terminus of VP22. To explore the application of such trafficking, we constructed plasmids expressing green fluorescent protein (GFP) fused to the C-terminus of either wild-type VP22 or a 160 amino acid peptide from VP22. In vitro studies showed that the majority of both fused proteins were localized to the nuclei of transfected cells. Quantitative analysis of GFP-positive cells, however, showed no significant increase in intercellular protein trafficking for cells transfected with either fusion protein compared with a lacZ-expressing plasmid. Our results suggest that the use of HSV-1 VP22 for mediating intercellular trafficking of transgene products is limited.
- Subjects
GREEN fluorescent protein; CELL communication; HERPES simplex virus; GENE transfection
- Publication
Gene Therapy, 1998, Vol 5, Issue 10, p1420
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3300741