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- Title
Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep TBXT Heterozygotes.
- Authors
Yang, Guang; Wang, Caiyun; Su, Hong; Wang, Daqing; Dou, Aolie; Chen, Lu; Ma, Teng; Liu, Moning; Su, Jie; Xu, Xiaojing; Yang, Yanyan; He, Tingyi; Li, Xihe; Song, Yongli; Cao, Guifang
- Abstract
Simple Summary: TBXT (c.333G > C; c.334G > T) has been identified as a molecular genetic marker in short-tailed sheep. This paper describes a high-resolution melting (HRM) analysis using unlabeled probes and asymmetric PCR for the detection of genetic variants of TBXT in short-tailed sheep populations. The detection results of this method are consistent with those of Sanger sequencing and can help farmers with marker-assisted breeding. The short-tailed phenotype has long been considered one of the best traits for population genetic improvement in sheep breeding. In short-tailed sheep, not only is tail fat eliminated but also the pubic area is exposed due to the lack of a tail covering, giving them an advantage in reproduction. Recent studies have shown that two linked mutations in sheep TBXT at nucleotides 333 and 334 are associated with the short-tailed phenotype. In the population of short-tailed sheep, several heterozygous mutants of this gene are found. In our research, we used high-resolution melting (HRM) to identify homozygous and heterozygous genotypes in a flock of short-tailed sheep and compared the results with those of Sanger sequencing, which were identical. This demonstrates that our established HRM method, a rapid and inexpensive genotyping method, can be used to identify homozygous and heterozygous individuals in short-tailed sheep flocks.
- Subjects
SHEEP breeding; SHEEP; SHEEP breeds; GENETIC carriers; GENETIC markers; MELTING
- Publication
Animals (2076-2615), 2022, Vol 12, Issue 6, p792
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani12060792