We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Gene expression profiling of M ycobacterium tuberculosis Lipoarabinomannan-treated macrophages: A role of the Bcl-2 family member A1 in inhibition of apoptosis in mycobacteria-infected macrophages.
- Authors
Halder, Priyanka; Kumar, Ranjeet; Jana, Kuladip; Chakraborty, Sohini; Ghosh, Zhumur; Kundu, Manikuntala; Basu, Joyoti
- Abstract
Macrophages play an important role in the establishment of infection by intracellular pathogens. Mycobacterium tuberculosis is known to inhibit apoptosis and to downregulate immune responses of host cells using various strategies, including activation of peroxisome proliferator-activated receptor (PPAR)γ. Mannose-capped lipoarabinomannan (ManLAM) is one of the known bacterial effectors that plays a role in subversion of host immunity and activation of PPARγ. Here, we have used an unbiased global gene expression profiling approach to understand ( a) how ManLAM regulates host cell immune responses and ( b) the role of PPARγ in modulating ManLAM-induced host cell signaling. We have demonstrated that ManLAM-dependent inhibition of macrophage apoptosis is mediated by the upregulation of the antiapoptotic B-cell CLL/ lymphoma 2 (Bcl2) family member A1. Our in silico analyses suggested that ManLAM-mediated PPARγ signaling is linked to important functions such as phagocytosis, cytoskeleton remodeling, cell survival, and autophagy. We have validated that ManLAM upregulates signal transducer and activator of transcription ( STAT5) α, an important transcriptional regulator of cell survival in a PPARγ-dependent manner. © 2015 IUBMB Life, 67(9):726-736, 2015
- Subjects
MACROPHAGES; MYCOBACTERIUM tuberculosis; APOPTOSIS; IMMUNE response; CELLULAR signal transduction; GENETICS
- Publication
IUBMB Life, 2015, Vol 67, Issue 9, p726
- ISSN
1521-6543
- Publication type
Article
- DOI
10.1002/iub.1430