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- Title
Cellular hnRNP A1 Interacts with Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus and Impairs Viral Replication.
- Authors
Li, Zhonghua; Zeng, Wei; Ye, Shiyi; Lv, Jian; Nie, Axiu; Zhang, Bingzhou; Sun, Yumei; Han, Heyou; He, Qigai
- Abstract
Abstract: The nucleocapsid (N) protein is a major structural component of porcine epidemic diarrhea virus (PEDV), which is predicted to be a multifunctional protein in viral replication. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a cellular protein participating in the splicing of pre-mRNA in the nucleus and translation regulation in the cytoplasm. According to our previous proteomic study about PEDV infection in vivo, hnRNP A1 was thought to be a cellular factor influencing PEDV replication. In this report, PEDV N protein was discovered to colocalize with cellular hnRNP A1 in perinuclear region of PEDV infected cells. Co-immunoprecipitation (CO-IP) results clearly demonstrated that PEDV N protein could bind to human hnRNP A1. Replication of PEDV was inhibited by silencing the expression of hnRNP A1 in CCL-81 cells, suggesting the positive effect of hnRNP A1 on PEDV infection.
- Subjects
SYNCRIP protein; NUCLEOCAPSID structure; PORCINE epidemic diarrhea virus; VIRAL replication; RIBOSOMES; IMMUNOPRECIPITATION
- Publication
Viruses (1999-4915), 2018, Vol 10, Issue 3, p127
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v10030127