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- Title
A putative terpene cyclase gene (CcPtc1) is required for fungal development and virulence in Cytospora chrysosperma.
- Authors
Yuchen Yang; Lu Yu; Xiaolin Qiu; Dianguang Xiong; Chengming Tian
- Abstract
Cytospora chrysosperma is a destructive plant pathogenic fungus, which causes canker disease on numerous woody plants. However, knowledge concerning the interaction between C. chrysosperma and its host remains limited. Secondary metabolites produced by phytopathogens often play important roles in their virulence. Terpene cyclases (TC), polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) are the key components for the synthesis of secondary metabolites. Here, we characterized the functions of a putative terpene type secondary metabolite biosynthetic core gene CcPtc1 in C. chrysosperma, which was significantly up-regulated in the early stages of infection. Importantly, deletion of CcPtc1 greatly reduced fungal virulence to the poplar twigs and they also showed significantly reduced fungal growth and conidiation compared with the wild-type (WT) strain. Furthermore, toxicity test of the crude extraction from each strain showed that the toxicity of crude extraction secreted by 1CcPtc1 were strongly compromised in comparison with the WT strain. Subsequently, the untargeted metabolomics analyses between 1CcPtc1 mutant and WT strain were conducted, which revealed 193 significantly different abundant metabolites (DAMs) in1CcPtc1 mutant compared to the WT strain, including 90 significantly downregulated metabolites and 103 significantly upregulated metabolites, respectively. Among them, four key metabolic pathways that reported to be important for fungal virulence were enriched, including pantothenate and coenzyme A (CoA) biosynthesis. Moreover, we also detected significant alterations in a series of terpenoids, among which (+)-ar-turmerone, pulegone, ethyl chrysanthemumate, and genipin were significantly downregulated, while cuminaldehyde and (±)-abscisic acid were significantly upregulated. In conclusion, our results demonstrated that CcPtc1 acts as a virulence-related secondary metabolism factor and provides new insights into the pathogenesis of C. chrysosperma.
- Subjects
FUNGAL virulence; CANKER (Plant disease); NONRIBOSOMAL peptide synthetases; METABOLITES; POLYKETIDE synthases; MENTHENONE; TERPENES
- Publication
Frontiers in Microbiology, 2023, Vol 14, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2023.1084828