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- Title
Mouse Ribosomal RNA Genes Contain Multiple Differentially Regulated Variants.
- Authors
Hung Tseng; Weichin Chou; Junwen Wang; Xiaohong Zhang; Shengliang Zhang; Schultz, Richard M.
- Abstract
Previous cytogenetic studies suggest that various rDNA chromosomal loci are not equally active in different cell types. Consistent with this variability, rDNA polymorphism is well documented in human and mouse. However, attempts to identify molecularly rDNA variant types, which are regulated individually (i.e., independent of other rDNA variants) and tissue-specifically, have not been successful. We report here the molecular cloning and characterization of seven mouse rDNA variants (v-rDNA). The identification of these v-rDNAs was based on restriction fragment length polymorphisms (RFLPs), which are conserved among individuals and mouse strains. The total copy number of the identified variants is less than 100 and the copy number of each individual variant ranges from 4 to 15. Sequence analysis of the cloned v-rDNA identified variant-specific single nucleotide polymorphisms (SNPs) in the transcribed region. These SNPs were used to develop a set of variant-specific PCR assays, which permitted analysis of the v-rDNAs' expression profiles in various tissues. These profiles show that three v-rDNAs are expressed in all tissues (constitutively active), two are expressed in some tissues (selectively active), and two are not expressed (silent). These expression profiles were observed in six individuals from three mouse strains, suggesting the pattern is not randomly determined. Thus, the mouse rDNA array likely consists of genetically distinct variants, and some are regulated tissue-specifically. Our results provide the first molecular evidence for cell-typespecific regulation of a subset of rDNA.
- Subjects
RECOMBINANT DNA; GENETIC polymorphisms; HUMAN beings; MICE; MOLECULAR cloning; RESTRICTION fragment length polymorphisms; POLYMERASE chain reaction; DIFFERENCES; CHROMOSOMES
- Publication
PLoS ONE, 2008, Vol 3, Issue 3, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0001843