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- Title
Técnica para aislamiento de bacteriófagos específicos para E.coli DH5α a partir de aguas residuales.
- Authors
Gaviria A., Gabriel; González De S., María; Castaño O., Jhon
- Abstract
Objetive. To develop an isolation technique for DH5α E. coli specific bacteriophages in wastewater. Materials and methods. The 1601 method of the Environmental Protection Agency of the United States of America and an wastewater enterovirus obtaining method were used as a reference. During the development of the protocol, multiple tests were performed using the T4 bacteriophage as positive control and the bacteriophage isolates obtained from wastewater. Results. Plaque-forming units (PFU) were observed; tittering of bacteriophages present in each of the DH5α E. coli cultures was obtained; the relationships between the quantification of PFU in the control and experimental groups, and their corresponding PFU characteristics were determined for each of the experiments. Conclusions. It was possible to establish a microbiological protocol for the isolation of bacteriophages specific for DH5α E. coli.
- Subjects
UNITED States; BACTERIOPHAGES; ESCHERICHIA coli; WASTEWATER treatment; UNITED States. Environmental Protection Agency. Office of Water
- Publication
Revista MVZ Córdoba, 2012, Vol 17, Issue 1, p2852
- ISSN
0122-0268
- Publication type
Article
- DOI
10.21897/rmvz.253