We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Formulation of Culture Medium with Agroindustrial Waste for β-Galactosidase Production from Kluyveromyces marxianus ATCC 16045.
- Authors
Braga, Anna; Gomes, Patrícia; Kalil, Susana
- Abstract
Seven strains of the genus Kluyveromyces were screened for β-galactosidase activity and Kluyveromyces marxianus ATCC 16045 was selected as the best enzyme producer for culture medium optimization. The production of β-galactosidase by submerged cultivation was evaluated using a factorial design and response surface methodology. The culture medium containing whey and parboiled rice effluent was formulated to maximize the production of β-galactosidase. The effects of the initial pH and the concentrations of whey lactose, peptone, (NH)SO, yeast extract, and parboiled rice effluent on enzyme production were studied using a 2 fractional design. A CCRD (24 trials plus axial and central points) was used for the four variables selected from the fractional design (lactose, peptone, (NH)SO and yeast extract), with β-galactosidase activity as the response. The optimum conditions established for production were a whey (lactose) concentration of 120 g/L, a yeast extract concentration of 5 g/L, a peptone concentration of 15 g/L, a (NH)SO concentration of 15 g/L, a parboiled rice effluent concentration of 30 g/L, and a pH value of 4.0. Under these conditions, the highest enzymatic activity of 10.4 U/mL was measured, being 9.5-9.7 as the values predicted by the proposed model, showing an enzymatic activity increase of 30% using alternative sources of lactose and nitrogen for β-galactosidase production.
- Subjects
AGRICULTURAL industries; AGRICULTURAL wastes; GALACTOSIDASES; KLUYVEROMYCES marxianus; PARBOILED rice; WHEY; YEAST extract
- Publication
Food & Bioprocess Technology, 2012, Vol 5, Issue 5, p1653
- ISSN
1935-5130
- Publication type
Article
- DOI
10.1007/s11947-011-0511-0