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- Title
Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli ( Capsicum annuum L.).
- Authors
Naresh, P.; Krishna Reddy, M.; Reddy, Anand; Lavanya, B.; Lakshmana Reddy, D.; Madhavi Reddy, K.
- Abstract
Viruses are serious threat to chilli crop production worldwide. Resistance screening against several viruses resulted in identifying a multiple virus resistant genotype 'IHR 2451'. Degenerate primers based on the conserved regions between P-Loop and GLPL of Resistance genes (R-genes) were used to amplify nucleotide binding sites (NBS)-encoding regions from genotype 'IHR 2451'. Alignment of deduced amino acid sequences and phylogenetic analyses of isolated sequences distinguished into two groups representing toll interleukin-1 receptor (TIR) and non-TIR, and different families within the group confirming the hypotheses that dicots have both the types of NBS-LRR genes. The alignment of deduced amino acid sequences revealed conservation of subdomains P-loop, RNBS-A, kinase2, RNBS-B, and GLPL. The distinctive five RGAs showing specific conserved motifs were subjected to BLASTp and indicated high homology at deduced amino acid level with R genes identified such as Pvr9 gene for potyvirus resistance, putative late blight resistance protein homolog R1B-23 and other disease resistance genes suggesting high correlation with resistance to different pathogens. These pepper RGAs could be regarded as candidate sequences of resistant genes for marker development.
- Subjects
PEPPER genetics; BIODIVERSITY; LAMINATED root rot; NATURAL immunity; PLANT resistance to viruses
- Publication
3 Biotech, 2017, Vol 7, Issue 2, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-017-0720-y