We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Molecular modeling and expression analysis of a MADS- box cDNA from mango ( Mangifera indica L.).
- Authors
Pacheco-Sánchez, Magda; Contreras-Vergara, Carmen; Hernandez-Navarro, Eduardo; Yepiz-Plascencia, Gloria; Martínez-Téllez, Miguel; Casas-Flores, Sergio; Arvizu-Flores, Aldo; Islas-Osuna, Maria
- Abstract
MADS- box genes are a large family of transcription factors initially discovered for their role during development of flowers and fruits. The MADS-box transcription factors from animals have been studied by X-ray protein crystallography but those from plants remain to be studied. In this work, a MADS- box cDNA from mango encoding a protein of 254 residues was obtained and compared. Based on phylogenetic analysis, it is proposed that the MADS-box transcription factor expressed in mango fruit (MiMADS1) belongs to the SEP clade of MADS-box proteins. MiMADS1 mRNA steady-state levels did not changed during mango fruit development and were up-regulated, when mango fruits reached physiological maturity as assessed by qRT-PCR. Thus, MiMADS1 could have a role during development and ripening of this fruit. The theoretical structural model of MiMADS1 showed the DNA-binding domain folding bound to a double-stranded DNA. Therefore, MiMADS1 is an interesting model for understanding DNA-binding for transcriptional regulation.
- Publication
3 Biotech, 2014, Vol 4, Issue 4, p357
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-013-0162-0