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- Title
IDENTIFICATION OF SSR MARKERS FOR HYBRIDITY AND SEED GENETIC PURITY TESTING IN SUNFLOWER (Helianthus annuus L.).
- Authors
Pallavi, H.M.; Gowda, Rame; Shadakshari, Y.G.; Bhanuprakash, K.; Vishwanath, K.
- Abstract
The genuineness of a hybrid is one of the most important characteristics of good quality seed. In order to identify pure hybrid and pollen shedders/ offtypes an investigation was performed to identify an ideal SSR marker. 58 primer pairs were screened to identify the specific marker associated with each hybrid and parental lines. Hybrid KBSH-44 could be clearly identified by using ORS 309 and ORS 170, based on the banding pattern resolved on polyacrylamide gel (6%). The complementary banding pattern of both parents made a way to identify the hybrid. ORS 309 amplified allele size at 250 bp was specific to female parent (CMS-17A) and 230 bp was specific to male parent (RHA 95- C-1). These two bands of allele size 230 and 250 bp were found only in hybrid KBSH-44. Another SSR primer ORS 170 was able to distinguish the hybrid KBSH-44 by amplifying allele of size 230 bp a female specific (CMS-17A) allele and 200 bp amplicon a male specific allele (RHA 95-C-1). SSR primer ORS 811 found specific to identify KBSH-53 and it amplified allele of size 270 bp in its female parent (CMS-53A) and allele size of 230 bp in its pollen parent (RHA 95-C-1). The hybrid has both the alleles from its parents at 270 and 230 bp.
- Subjects
SUNFLOWER seeds; SUNFLOWER genetics; SEED quality; MICROSATELLITE repeats; PLANT breeding; GENETIC markers in plants; POLLEN
- Publication
Helia, 2011, Vol 34, Issue 54, p59
- ISSN
1018-1806
- Publication type
Article
- DOI
10.2298/HEL1154059P