We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Antioxidant and antibacterial properties of Monodora myristica (Calabash nutmeg) seed protein hydrolysates.
- Authors
Osukoya, Olukemi Adetutu; Adewale, Olusola Bolaji; Falade, Adeoluwa Emmanuel; Afolabi, Olakunle Bamikole; Awe, Joseph Obabiolorunkosi; Obafemi, Tajudeen Olabisi; Kuku, Adenike
- Abstract
The purpose of this study was to investigate some of the properties (antioxidant, antibacterial and functional properties) of Monodora myristica seed protein hydrolysates. Monodora myristica protein was extracted using alkaline solubilization, followed by acid precipitation to its isoelectric point. Monodora myristica seed protein isolate was hydrolyzed using three enzymes separately (pepsin, trypsin and pancreatin). Antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging and ferrous ion (Fe2+)-chelating) were determined using standard procedures. Antibacterial activities of protein hydrolysates were done using zone of inhibition test. All protein hydrolysates of Monodora myristica had significantly high DPPH radical scavenging and Fe2+-chelating properties and were soluble over a wide pH range with more than 30% solubility. Pepsin hydrolysate had the highest foam expansion and stability of 117.4% and 98%, respectively and trypsin hydrolysate showed the lowest foam expansion and stability of 97.8% and 78.2%, respectively. Only proteins hydrolyzed with pepsin (regardless of the duration of hydrolysis) demonstrated potent antibacterial activity on test bacteria (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Proteus vulgaris). It could be deduced from this study that enzymatic hydrolysates of Monodora myristica protein has potential application among functional foods, and may be a promising source of natural antioxidants and antibacterial drugs.
- Subjects
MONODORA; ANTIOXIDANT analysis; ANTIBACTERIAL agents; SEED proteins; PROTEIN hydrolysates
- Publication
Journal of Food Measurement & Characterization, 2021, Vol 15, Issue 3, p2854
- ISSN
2193-4126
- Publication type
Article
- DOI
10.1007/s11694-021-00871-4