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- Title
Identification of Resting Human T and B Lymphocytes by Acid α-Naphthyl Acetate Esterase Staining Combined with Rosette Formation with Staphylococcus aureus Strain Cowan 1.
- Authors
Ranki, A.; Tötterman, T. H.; Häyry, P.
- Abstract
We describe a method enabling the identification of both lymphocyte thus and morphology from a single microscopic slide. As a marker for B tells we used surface immunoglobulin. The surface-Ig-carrying cells were rosetted after polyvalent anti-Ig treatment with Staphylococcus aureus strain Cowan 1 (StaCw) and the tells were cytocentrifuged onto a microscope slide. The lymphocytes forming rosettes with StaCw were identical with cells expressing surface Ig studied by fluorescein-isothiocyanate-conjugated anti-Ig. As a marker for T cells we used the acid α-naphthyl acetate esterase (ANAE) histochemical marker. The cell smears were first stained for ANAE and subsequently counterstained to distinguish also cell morphology. The ANAH-marker-carrying cells were all included in the population of lymphocytes forming rosettes with sheep erythrocytes. Thus both T and B lymphocytes could be simultaneously identified from a single microscopic slide, and we therefore recommend the method for routine clinical work.
- Subjects
T cells; B cells; LYMPHOCYTES; STAPHYLOCOCCUS; LEUCOCYTES; ERYTHROCYTES; SHEEP as laboratory animals
- Publication
Scandinavian Journal of Immunology, 1976, Vol 5, Issue 10, p1129
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1976.tb00254.x