We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of Treating Immune Lymphocytes with a Proteolytic Enzyme (Papain): Reduction in Immune Potential and Recovery after Incubation.
- Authors
Cross, A. M.
- Abstract
Immune spleen cells were treated with papain, washed, and boosted with homologous antigen (4-hydroxy-3-iodo-5-nitrophenylacetic acid coupled to chicken globulin) for 1 hr at 4°C. When transferred to irradiated mice, they produced up to 20-fold less anti-hapten antibody than non-papain-treated cells. The lymphocytes could recover their ability to respond if incubated at 37°C after papain treatment but before antigen boosting. Incubation for 30 min at 37°C gave complete or almost complete recovery. Although papain usually reduced the response, in 31% of experiments the reduction was not significant. Possible reasons for this variability are discussed.
- Subjects
IMMUNITY; LYMPHOCYTES; LEUCOCYTES; PROTEOLYTIC enzymes; HYDROLASES; ANTIGENS; GLOBULINS
- Publication
Scandinavian Journal of Immunology, 1975, Vol 4, Issue 3, p235
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1975.tb02622.x