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- Title
Generation of T Memory Cells in One-Way Mixed Lymphocyte Culture II. Anamnestic Responses of 'Secondary' Lymphocytes.
- Authors
Häyry, P.; Andersson, L. C.
- Abstract
The peak of blast response in primary, one-way mixed lymphocyte culture of CBA plus DBA/2m took place on culture day 6 to 7, whereafter the number of blasts per culture declined. Concomitanlly with the disappearance of blast cells from these cultures, the number of lymphocytes increased, indicating that a certain number of blasts reverts back to lymphocytes ("secondary lymphocytes"). The number of antigen- reactive tells in primary mixed culture (quantitated by hydroxyurea block) was on the order of 3% to 5%, whereas when the same cultures were reprimed with the original antigen, the number of antigen-reactive cells was on the order of 90%. Thus most lymphocytes surviving prolonged primary mixed lymphocyte culture are in fact secondary lymphocytes--that is, blast-derived. The secondary lymphocytes thus obtained responded promptly and in a biphasic manner to the original and H-2 cross-reactive stimulator cells in secondary mixed culture. The first, major response peak took place on culture day 2 to 3 and the second, minor peak on culture day 5 to 6. Although the secondary lymphocytes were sensitive to anti-0 plus complement, they did not respond to phytohemagglutinin. The secondary lymphocytes were themselves cytotoxic to relevant allogeneic target cells in vitro, and they were promptly reinduced to maximal cytotoxicity by a second priming with the original antigen. We suggest that these functions are characteristic to T memory cells primed against histoincompatible cells.
- Subjects
LEUCOCYTES; LYMPHOCYTES; T cells; CELL-mediated lympholysis; FOA-Kurlov cells; B cells
- Publication
Scandinavian Journal of Immunology, 1974, Vol 3, Issue 6, p823
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1974.tb01318.x