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- Title
Functional Disturbance of Naive T Lymphocytes in Very High IgE Producers: Depletion of lnterleukin-4-lnduced lnterleukin-4-Producing Cells.
- Authors
Ochi, Hiroshi; Tanaka, Toshio; Katada, Yoshinori; Naka, Tetsuji; Aitani, Masakazu; Hashimoto, Shoji; Maeda, Keiji; Toyoshima, Kyoichiro; Igarashi, Tsuyoshi; Suemura, Masaki; Kishimoto, Tadamitsu
- Abstract
We examined the capacity of T cells from normal individuals and allergic patients with very high IgE to differentiate into interleukin-4 (IL-4)-producing cells in vitro. T cells incubated with anti-CD3 monoclonal antibody plus IL-4 or plus anti-IL-4 antibody in the presence of antigen-presenting cells for 7 days were restimulated and their capacity to express IL-4 mRNA was examined by RT-PCR. In T cells from normal individuals, there was a marked increase in the expression of IL-4 mRNA following the addition of IL-4. After fractionation of normal T cells into naive T (CD45RA+) and memory T (CD45RO+) cells, induction of the increase of IL-4 mRNA was restricted to the naive T cell population. In contrast, in T cells from allergic patients, the stimulation of whole or naive T cells with anti-CD3 monoclonal antibody in the presence of IL-4 induced much less IL-4 mRNA. These findings suggest the presence of a functional abnormality in IL-4-dependent development of IL-4-producing T cells in the peripheral-blood naive T cells from allergic patients. Copyright © 1996 S. Karger AG, Basel
- Publication
International Archives of Allergy & Immunology, 1996, Vol 109, Issue 3, p236
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000237244