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- Title
Cytokine Production by a New Undifferentiated Human Thyroid Carcinoma Cell Line, FB-1.
- Authors
FIORE, LISA; POLLINA, LUCA E.; FONTANINI, GABRIELLA; CASALONE, ROSARIO; BERLINGIERI, MARIA T.; GIANNINI, RICCARDO; PACINI, FURIO; MICCOLI, PAOLO; TONIOLO, ANTONIO; FUSCO, ALFREDO; BASOLO, FULVIO
- Abstract
human anaplastic thyroid cancer cell line FB-1, derived from a 68-yr-old woman who underwent surgery for anaplastic thyroid cancer, has been established. The spindlelike cells have been proliferating stably for more than 2 yr. Karyotype analysis shows many abnormalities and many marker chromosomes have been observed. Heterotransplant of FB-1 cells into severe combined immunodeficient mice has resulted in rapidly growing tumors classified as anaplastic carcinomas, although 50% have shown areas with a trabecular pattern. FB-1 cells failed to express messenger RNA for thyroglobulin; TSH-receptor; thyroperoxidase, and placental angiogenic growth factor. Conversely, PAX8 and thyroid transcription factor 1, whose expression is thyroid specific, was kept in an FB-1 cell line at a level comparable with that observed in normal thyroid tissue. In addition, the present cell line expressed high levels of messenger RNA for high-mobility group proteins (Y) and -C. The in vitro study revealed that FB-1 cells are able to produce high levels of interleukin (IL)-8 and medium amount of IL-6, whereas no release of IL-1-α, IL-1-β, and IL-4 was observed. No modulation of cell proliferation and DNA synthesis in FB-1 cells has been observed after the addition of exogenous IL-6.
- Publication
Journal of Clinical Endocrinology & Metabolism, 1997, Vol 82, Issue 12, p4094
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/jcem.82.12.4444