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- Title
The potential of organoids in toxicologic pathology: role of toxicologic pathologists in in vitro chemical hepatotoxicity assessment.
- Authors
Toshinori Yoshida; Mio Kobayashi; Suzuka Uomoto; Kanami Ohshima; Emika Hara; Yoshitaka Katoh; Naofumi Takahashi; Takanori Harada; Tatsuya Usui; Elbadawy, Mohamed; Makoto Shibutani
- Abstract
The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. Herein, we briefly discuss the current status of hepatoxicity assessment using human- and rat-derived hepatocytes; we focus on the liver organoid method, which has been extensively studied in recent years, and discuss how toxicologic pathologists can use their knowledge and experience to contribute to the development of in vitro chemical hepatotoxicity assessment methods for drugs, pesticides, and chemicals. We also propose how toxicological pathologists should assess toxicity regarding the putative distribution of undifferentiated and differentiated cells in the organoid when liver organoids are observed in hematoxylin and eosin-stained specimens. This was done while considering the usefulness and limitations of in vitro studies for toxicologic pathology assessment.
- Subjects
PATHOLOGISTS; HEPATOTOXICOLOGY; ORGANOIDS; PATHOLOGY; PESTICIDES; LIVER cells; ANIMAL experimentation; ANIMAL welfare
- Publication
Journal of Toxicologic Pathology, 2022, Vol 35, Issue 3, p225
- ISSN
0914-9198
- Publication type
Article
- DOI
10.1293/tox.2022-0017