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- Title
Cloning and characterization of the rhesus monkey nociceptin/orphanin FQ receptor.
- Authors
Koga, Kazumi; Ichikawa, Daisuke; Nambu, Hirohide; Azuma-Kanoh, Tomoko; Sakai, Naoko; Takaki-Kawagoe, Hiroko; Ozaki, Satoshi; Ohta, Hisashi
- Abstract
We succeeded in cloning the rhesus monkey nociceptin/orphanin FQ peptide (NOP) receptor. The nucleotide sequence and amino acid sequence of the rhesus monkey NOP receptor were 95.9% and 97.8%, respectively, identical to the human NOP receptor. There was no significant difference between the rhesus monkey NOP receptor and the human NOP receptor in the binding affinity of [125I] [Thy14]nociceptin and the binding of [35S]guanosine 5'-O-(γ thio)triphospate ([35S]GTPγS) stimulated by nociceptin/orphanin FQ (N/OFQ). A selective NOP receptor antagonist, 1-[(3R,4R)-l-cyclooctylmethyl-3-hydroxymethyl-4-piperldyl]-3-ethyl-l, 3-dihydro-2H-benzimidazol-2-one ((+)-J-113397) inhibited the [35S]GTPγS binding activated by N/OFQ using the membrane of the rhesus monkey NOP receptor. The antagonistic activity of (+)-J-113397 to the rhesus monkey NOP receptor was comparable to that to the human NOP receptor. Thus, N/OFQ acts via activation of the NOP receptor in both human and rhesus monkeys without significant species differences.
- Subjects
CLONING; GENETIC engineering; RHESUS monkeys; PEPTIDES; NUCLEOTIDE sequence; AMINO acid sequence; GUANOSINE triphosphate
- Publication
Genes & Genetic Systems, 2009, Vol 84, Issue 5, p319
- ISSN
1341-7568
- Publication type
Article
- DOI
10.1266/ggs.84.319