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- Title
Triiodothyronine stimulates glucose transport in bone cells.
- Authors
Zoidis, Evangelos; Ghirlanda-Keller, Claudia; Schmid, Christoph
- Abstract
Thyroid hormones increase energy expenditure and bone turnover in vivo. To study whether 3,3′,5-triiodo-l-thyronine (T) stimulates the uptake of glucose in osteoblastic cells, PyMS (a cell line derived from rat bone) cells were kept in serum-free culture medium and treated with T. We measured [1-C]-2-deoxy- d-glucose (2DG) uptake and looked for expression of the high-affinity glucose transporters GLUT1 and GLUT3 by northern and western analysis. T did not influence the cell number but slightly (1.3-fold) increased the protein content of the cell cultures. 2DG uptake was low in serum-deprived cell cultures and was increased by T (up to 2.5-fold at 1 nmol l after 4 days) in a dose- and time-dependent manner. Triiodothyronine at 1 nmol l increased GLUT1 and GLUT3 abundance in membranes. Therefore, increased glucose uptake induced by T in osteoblasts may be mediated by the known high-affinity glucose transporters GLUT1 and GLUT3.
- Subjects
THYROID hormones; GLUCOSE; CELL culture; THYRONINES; PROTEIN analysis
- Publication
Endocrine (1355008X), 2012, Vol 41, Issue 3, p501
- ISSN
1355-008X
- Publication type
Article
- DOI
10.1007/s12020-012-9594-2