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- Title
Effect of a high surface-to-volume ratio on fluorescence-based assays.
- Authors
Kwapiszewski, Radoslaw; Ziolkowska, Karina; Zukowski, Kamil; Chudy, Michal; Dybko, Artur; Brzozka, Zbigniew
- Abstract
In the work discussed in this paper, the effect of a high surface-to-volume ratio of a microfluidic detection cell on fluorescence quenching was studied. It was found that modification of the geometry of a microchannel can provide a wider linear range. This is a phenomenon which should be taken into consideration when microfluidic systems with fluorescence detection are developed. The dependence of the linear range for fluorescein on the surface-to-volume ratio was determined. Both fluorescence inner-filter effects and concentration self-quenching were taken into consideration. It was found that inner-filter effects have little effect on the extent of the linear range on the microscale. [Figure not available: see fulltext.]
- Subjects
MICROFLUIDICS; FLUORESCENCE; PHTHALEINS; RADIOACTIVITY; FLUORESCEIN
- Publication
Analytical & Bioanalytical Chemistry, 2012, Vol 403, Issue 1, p151
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-012-5770-8