We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of efficient, cost-effective in vitro micropropagation technique for threatened ethnomedicinal plant Clerodendrum indicum (L.) O. Kuntze.
- Authors
Kundu, Ashutosh; Sahani, Bikram; Adhikary, Rajsekhar; Chakraborty, Anindita; Seal, Tapan; Mandal, Vivekananda
- Abstract
Clerodendrum indicum (L.) O. Kuntze (Verbenaceae) is a threatened ethnomedicinal plant with many bioactive secondary metabolites that could alleviate chronic diseases like cough, asthma, jaundice, leprosy, syphilitic rheumatism, and septic wounds. Their natural growth has been severely challenged due to habitat loss and massive exploitation for medical applications, leading to the threatened status. Therefore, an in vitro micropropagation technique has been trialled to be used for eco-restoration and metabolite exploitation. Micropropagation via direct and indirect shoot organogenesis had been established from the different explants of this plant. Murashige and Skoog (MS) media supplemented with variable concentrations of NAA, IAA, and BAP produced callus, organogenesis and whole plant. The study revealed that nodal explants resulted in more significant responses than others. The shoot and root regeneration through callus was observed in the MS media supplemented with 4.0 mg/L 6-BA and 0.5 mg/L NAA with an 84% response rate after two weeks of incubation and an average 5.6 number of shoots per callus. Only root and shoot regeneration was observed using half-strength MS media with 2.0 mg/L 6-BA and 1.0 mg/L NAA and 6.0 mg/L 6-BA and 0.5 mg/L NAA, respectively, after two weeks. The plantlets’ acclimatization had an average survival rate of 80%. The Soxhlet and microwave-assisted microscale extractions were done using dichloromethane solvent for HPLC quantitation of the secondary metabolites. The study revealed that the HPLC analysis of the plant extracts demonstrated the occurrence of 18 different phenolic acids and flavonoids in the ex-situ plant organs, while the in vitro plant organs only had 04 compounds, such as kaempferol (1.533 µg/mg), apigenin (0.871 µg/mg), ferulic acid (0.192 µg/mg), and syringic acid (0.012 µg/mg). These in-vitro regenerated plants by direct shoot organogenesis and callus induction methods might aid in harvesting a bulk amount of secondary metabolites without destroying the native habitat. Thus, these methods would lead to sustainable environmental restoration.
- Publication
Plant Cell, Tissue & Organ Culture, 2024, Vol 157, Issue 2, p1
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-024-02744-2