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- Title
Transfer of a Dwarf Gene from Brassica rapa to Oilseed B. napus, Effects on Agronomic Traits, and Development of a ‘Perfect’ Marker for Selection.
- Authors
Muangprom, Amorntip; Mauriera, Ivan; Osborn, Thomas
- Abstract
Lodging can be a serious problem in oilseed Brassica napus. Dwarf genes have been used to reduce lodging in other crops, and their use in wheat and rice was a major factor in the success of the ‘Green Revolution’. We previously reported on a single, semi-dominant, gibberellin insensitive dwarf mutant of B. rapa ( Brrga1-d), and provided evidence for homology of this gene to the wheat ‘Green Revolution’ dwarf gene. In this paper, we report using interspecific hybridization of B. rapa and B. oleracea and embryo rescue to resynthesize B. napus containing the Brrga1-d dwarf gene. The dwarf gene was backcrossed into two parents of a commercial hybrid combination and evaluated as inbred and hybrid lines in field experiments. The Brrga1-d gene reduced plant height and lodging in inbred and hybrid lines of B. napus, even when present as a single dose in heterozygous genotypes. Seed yields of inbred lines homozygous for Brrga1-d were reduced compared to near isogenic wild-type inbreds, presumably due to damage by insects caused by a slight delay in flowering time. However, yields of hybrids that were homozygous or heterozygous for Brrga1-d were similar to those of near isogenic wild-type hybrids. In addition, we report on a ‘perfect’ PCR marker for selection of the Brrga1-d gene that is based on detecting the nucleotide mutation causing the dwarf phenotype.
- Subjects
BRASSICA; OILSEEDS; PLANT genetics; WHEAT; RICE; GIBBERELLINS
- Publication
Molecular Breeding, 2006, Vol 17, Issue 2, p101
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-005-3734-9