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- Title
Highly efficient genome editing in N. gerenzanensis using an inducible CRISPR/Cas9–RecA system.
- Authors
Yue, Xue; Xia, Tianyu; Wang, Shuai; Dong, Huijun; Li, Yongquan
- Abstract
Objective: To develop an inducible CRISPR/Cas9–Recombinase A (RecA) system to manipulate genes in Nonomuraea gerenzanensis effectively. Results: Compared with traditional homologous recombination, the inducible CRISPR/Cas9 system achieved 68.8% editing efficiency, whereas, with both the inducible Cas9 and the overexpressed RecA, the efficiency of the combined genome editing system reached 100%. The dbv23-deleted mutant obtained by the inducible CRISPR/Cas9–RecA system was confirmed to produce more A40926 with an approximate yield of 200 mg L−1 than that of around 150 mg L−1 produced by the wild-type strain. Conclusions: This inducible CRISPR/Cas9–RecA system was successfully constructed and can be utilized as an efficient genome editing tool for Actinomyces able to shorten editing time simultaneously.
- Subjects
CRISPRS; GENOME editing; ACTINOMYCES
- Publication
Biotechnology Letters, 2020, Vol 42, Issue 9, p1699
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-020-02893-2