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- Title
Culture and Motion Analysis of Diatom Bacillaria paradoxa on a Microfluidic Platform.
- Authors
Cai, Jun; Chen, Mingli; Wang, Yu; Pan, Junfeng; Li, Aobo; Zhang, Deyuan
- Abstract
We proved the feasibility of using a microfluidic chip to culture diatom Bacillaria paradoxa, and analyzed the gliding characteristics of its self-organized colony in detail. The optimal cultivation parameters of B. paradoxa for the designed chip made with polydimethylsiloxane are as follows: the preferable cells injecting rate for keeping the cells alive is 0.2 mL/h, the initial cell density for fast reproduction is 5.5 × 10 cells/mL, and the optimal replacement period of culture medium is 4 days. B. paradoxa tends to form a colony during their growth, and the colony can glide with a steady period of 29 ± 3 s along its axial direction in a constant stream, the amplitude of the colony will not decay (e.g., 24 μm of two-cell colony at 1.1 mm/s flow rate), and the colony rapidly adjusts its direction of gliding to the direction of water flow. The successful culture of diatoms on a microfluidic platform may be used for biosensing chips and the creation of gasoline-producing diatom solar panels.
- Subjects
FRAGILARIACEAE; BACILLARIACEAE; MICROFLUIDICS; POLYDIMETHYLSILOXANE; GASOLINE; SOLAR cells
- Publication
Current Microbiology, 2013, Vol 67, Issue 6, p652
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-013-0413-7