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- Title
The GAL10 Gene is Located 40 kbp Away from the GAL7- GAL1 Region in the Yeast Kazachstania naganishii.
- Authors
Sugihara, Chisa; Hisatomi, Taisuke; Kodama, Takuya; Tsuboi, Michio
- Abstract
Of the genes involved in galactose metabolism, GAL7, GAL10, and GAL1 are tightly linked in this order on chromosome II in Saccharomyces cerevisiae. While several species of the order Saccharomycetales have similar gene organization, Kazachstania naganishii is unique, in which GAL7 and GAL1 are close to each other whereas GAL10 is substantially apart from them on chromosome XI. In this study, we inserted the recognition sequence of I- SceI homing-endonuclease into GAL10 and also into the intervening segment of GAL7-GAL1. By cleaving chromosome DNA of the gene-manipulated strain with I- SceI, we obtained evidence that chromosome XI (610 kbp) was replaced with three fragments (305, 265, and 40 kbp). Using appropriate probes, we further found that GAL10 was about 40 kbp apart from the GAL7-GAL1 cluster and that orientation of GAL10 was reversed comparing to the S. cerevisiae counter part. We, therefore, contend that comparison of the organization of the GAL cluster among Saccharomycetales is of importance to elucidate evolution of chromosomes and that the experimental scheme developed in this study is useful for this line of investigation.
- Subjects
SACCHAROMYCES cerevisiae; CELL nuclei; YEAST genetic engineering; GALACTOSE; ENDONUCLEASES
- Publication
Current Microbiology, 2011, Vol 63, Issue 4, p366
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-011-9988-z