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- Title
miRNA Stability in Frozen Plasma Samples.
- Authors
Balzano, Francesca; Deiana, Marta; Dei Giudici, Silvia; Oggiano, Annalisa; Baralla, Angela; Pasella, Sara; Mannu, Andrea; Pescatori, Mario; Porcu, Baingio; Fanciulli, Giuseppe; Zinellu, Angelo; Carru, Ciriaco; Deiana, Luca
- Abstract
MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing; we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at −80 °C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life.
- Subjects
MICRORNA; FROZEN blood; BLOOD plasma; GENETIC repressors; ETHYLENEDIAMINE
- Publication
Molecules, 2015, Vol 20, Issue 10, p19030
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules201019030