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- Title
An Extracorporeal Plasma Filtration Column with Specific Binding to Dengue Virions.
- Authors
Thonghong, Sasiwimon; Sinananpat, Pat; Chatsuwan, Tanittha; Srisawat, Nattachai; Insin, Numpon; Salakij, Saran; Boonyasuppayakorn, Siwaporn
- Abstract
Introduction: Dengue infection is a significant public health concern that no specific treatment is available. Extracorporeal plasmapheresis or plasma filtration is a treatment option for severe cases with complications. However, the commercial adsorption devices mainly contained size-exclusive porous beads to adsorb the plasma proteins nonselectively. Methods: We developed a 1:50 simulated circuit for dengue virus-specific adsorption using a flavivirus-specific (4G2) antibody entrapped into the alginate bead. Results: The reduction ratios of the viral titer after 3 h of continuous run were 63.00 ± 1.21%, and 93.97 ± 1.27% measured by reverse transcription qPCR, and plaque titration, respectively. No specific adsorption was observed with Enterovirus A71 or Escherichia coli bacteria. Conclusion: This study is a proof-of-concept for the potential use of a dengue virus-specific adsorption column in the 1:50 simulated circuit. The system could be applied to various clinical platforms by substituting target-specific antibodies.
- Subjects
DENGUE hemorrhagic fever; DENGUE; BLOOD proteins; TITERS; PUBLIC health
- Publication
Blood Purification, 2023, Vol 52, Issue 1, p60
- ISSN
0253-5068
- Publication type
Article
- DOI
10.1159/000524387