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- Title
Morphological and Molecular Investigation of Anaplasma Infection in Dromedary Camel (Camelus dromedarius) in Bushehr Province, Iran.
- Authors
Moradi, Zahra; Ebrahimzadeh, Elahe; Shayan, Parviz; Zarghami, Feisal
- Abstract
BACKGROUND: Anaplasmosis is caused by an obligate intracellular, gram-negative microorganism, which belongs to the family Anaplasmatacea and can be transmitted by ticks and other arthropods. OBJECTIVES: The present study aimed to investigate the status of Anaplasma spp. infection by microscopy and molecular methods in dromedary camels in Bushehr province, Iran. METHODS: A total of 139 blood samples were collected from dromedary camels in Bushehr province. Giemsa staining and nested-polymerase chain reaction (PCR) were conducted to detect Anaplasma infection in the dromedary camels. RESULTS: We found that 27 (19.4%) out of the total 139 blood samples were suspected for the presence of Anaplasma spp. by morphological study. The PCR and nested-PCR sequencing results showed 111 (80%) and 134 (96%) samples positive for Anaplasma spp. and BLAST search in NCBI GenBank presented 100% identity with Candidatus Anaplasma camelii. CONCLUSIONS: The molecular results presented the high frequency of Candidatus Anaplasma camelii in camels, in Bushehr city.
- Subjects
BUSHIHR (Iran); ANAPLASMOSIS; CAMELS; POLYMERASE chain reaction; MICROSCOPY
- Publication
Iranian Journal of Veterinary Medicine, 2021, Vol 15, Issue 3, p295
- ISSN
2251-8894
- Publication type
Article
- DOI
10.22059/IJVM.2020.306095.1005109