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- Title
Ca<sup>2+</sup> and EGF induce the differentiation of human embryo mesenchymal stem cells into epithelial-like cells.
- Authors
Wu, Minjuan; Zhou, Tong; Liu, Houqi
- Abstract
The mesenchymal to epithelial transition (MET) occurs in organ development and anti-tumorigenesis. We have investigated the effects of calcium (Ca2+) and epidermal growth factor (EGF) on human mesenchymal stem cell (hMSCs) differentiation into epithelial-like cells. hMSCs lost their biological characteristics after EGF transfection, and MET was achieved by adding 0.4 mmol Ca2+. Western blotting and immunofluorescence showed expression of EGF, keratin, keratin 19 (K19), β1-integrin, E-cadherin and phosphorylated focal adhesion kinase (p-FAK, Ser-910) increased in hMSCs infected with EGF and exposed to Ca2+, although Smad3 activation was downregulated. hMSCs co-stimulated with EGF transfection and Ca2+ can therefore differentiate into epithelial-like cells in vitro.
- Subjects
EPIDERMAL growth factor; MESENCHYMAL stem cells; CALCIUM; EPITHELIAL cells; HUMAN embryos; HUMAN embryology; CADHERINS
- Publication
Cell Biology International, 2015, Vol 39, Issue 7, p852
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.10398