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- Title
Rhabdastrellic acid-A inhibited PI3K/Akt pathway and induced apoptosis in human leukemia HL-60 cells
- Authors
Guo, Jing-Feng; Zhou, Jun-Min; Zhang, Yong; Deng, Rong; Liu, Jian-nan; Feng, Gong-Kan; Liu, Zong-Chao; Xiao, Ding-Jun; Deng, Song-Zhi; Zhu, Xiao-Feng
- Abstract
Abstract: Increasing evidence suggests that aberrant activation of PI3K/Akt is involved in many human cancers, and that inhibition of the PI3K/Akt pathway might be a promising strategy for cancer treatment. Our investigation indicates that Rhabdastrellic acid-A, an isomalabaricane triterpenoid isolated from the sponge, Rhabdastrella globostellata, inhibits proliferation of HL-60 cells with an IC50 value of 0.68μg/ml, and induces apoptosis. Rhabdastrellic acid-A also induces cleavage of the death substrate poly (ADP-ribose) polymerase (PARP) and caspase-3. Pretreatment of HL-60 cells with the caspase-3 specific inhibitor, DEVD-CHO, prevents Rhabdastrellic acid-A-induced DNA fragmentation and PARP cleavage. Activated PI3K and Akt significantly decreases after treatment with Rhabdastrellic acid-A in HL-60 cells. Expression levels of protein bcl-2, bax remain unchanged in response to Rhabdastrellic acid-A treatment in HL-60 cells. These results suggest that Rhabdastrellic acid-A inhibits PI3K/Akt pathway and induces caspase-3 dependent-apoptosis in HL-60 human leukemia cells.
- Subjects
APOPTOSIS; CELL death; LEUKEMIA; CANCER
- Publication
Cell Biology International, 2008, Vol 32, Issue 1, p48
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2007.08.009