We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Alpha-mangostin inhibits the migration and invasion of A549 lung cancer cells.
- Authors
Thi Kieu Trang Phan; Fahimeh Shahbazzadeh; Thi Thu Huong Pham; Takanori Kihara
- Abstract
Several studies have indicated that α-mangostin exerts anti-metastasis and antisubsistence effects on several types of cancer cells. Especially, the anti-metastatic effect of α-mangostin on cancer cells is a prospective function in cancer treatment. However, the metastasis process is complicated, and includes migration, invasion, intravasation, and extravasation; thus, the main target of anti-metastatic effect of α-mangostin is not known. In this study, we investigated the effects of α-mangostin on the invasion, subsistence, and migration of lung cancer cells under co-culture conditions with normal cells and regular mono-culture conditions. We found that α-mangostin killed the lung cancer and normal cells in a dose-dependent manner. Furthermore, the alteration in the surface mechanical properties of cells was examined by using atomic force microscopy. Although the α-mangostin concentrations of 5 and 10 µM did not affect the shortterm cell viability, they considerably decreased the Young's modulus of lung cancer cells implying a decline in cell surface actin cytoskeletal properties. Additionally, these concentrations of α-mangostin inhibited the migration of lung cancer cells. In coculture conditions (cancer cells with normal cells), the invasive activities of cancer cells on normal cells were discernibly observed, and was inhibited after treatment with 5 and 10 µM of α-mangostin. Taken together, α-mangostin suppressed the subsistence of lung cancer cells and displayed anti-metastatic activities by inhibiting the migration and invasion, and reducing the actin cytoskeleton of cancer cells. Our findings suggest that α-mangostin could be a potential therapeutic agent for cancer treatment.
- Subjects
CANCER cell migration; CANCER cells; LUNG cancer; CELLULAR mechanics; ATOMIC force microscopy; YOUNG'S modulus
- Publication
PeerJ, 2018, p1
- ISSN
2167-8359
- Publication type
Article
- DOI
10.7717/peerj.5027