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- Title
Preliminary Structural Study of Inactivated Yellow Fever Virus.
- Authors
Pichkur, E. B.; Samygina, V. R.; Ivanova, A. L.; Fedotov, A. Yu.; Ivanov, A. P.; Khvatov, E. V.; Ishmukhametov, A. A.; Vorovich, M. F.
- Abstract
Here we present a protocol for the isolation, purification, and inactivation of the yellow fever virus (YFV) aimed to facilitate high-resolution structure determination using cryo-electron microscopy (cryo-EM). Cryo-EM studies require the concentration of viral particles to be at least 1012 particle/mL. The concentration of the sample was evaluated by a combination of methods, including the plaque-forming assay, spectrophotometry, and protein polyacrylamide gel electrophoresis. High purity of the sample was confirmed by electrophoresis and transmission electron microscopy. The cryo-EM data set was collected and the 2D classification of viral particles was performed.
- Subjects
YELLOW fever; PHYTOPLASMAS; POLYACRYLAMIDE gel electrophoresis; TRANSMISSION electron microscopy
- Publication
Crystallography Reports, 2020, Vol 65, Issue 6, p915
- ISSN
1063-7745
- Publication type
Article
- DOI
10.1134/S1063774520060267