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- Title
Challenges in Direct Detection of Flaviviruses: A Review.
- Authors
Dias, Bruna de Paula; Barbosa, Camila Cavadas; Ferreira, Cyntia Silva; Mayra Soares Alves dos Santos, Samara; Arrieta, Orlando Alfredo Pineda; Malta, Wellington Carvalho; Gomes, Maria Laura Maximiano Dias; Alves e Silva, Mariela; Fonseca, Júlia de Matos; Borges, Lysandro Pinto; Silva, Breno de Mello
- Abstract
Arthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of interest to public health. Given the importance of early and accurate diagnosis, this review analyzes the methods of direct detection of flaviviruses, such as reverse transcription loop-mediated isothermal amplification, microfluidics, localized surface plasmon resonance, and surface-enhanced Raman scattering, and presents the advantages, disadvantages, and detection limits identified in studies reported in the literature for each methodology. Among the different methods available, it is essential to balance four fundamental indicators to determine the ideal test: good sensitivity, high specificity, low false positive rate, and rapid results. Among the methods analyzed, reverse transcription loop-mediated isothermal amplification stands out, owing to result availability within a few minutes, with good sensitivity and specificity; in addition, it is the best-characterized methodology.
- Subjects
ARBOVIRUSES; FLAVIVIRUSES; SERS spectroscopy; SURFACE plasmon resonance; MOSQUITOES; AEDES aegypti; PUBLIC interest; DETECTION limit
- Publication
Pathogens, 2023, Vol 12, Issue 5, p643
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens12050643