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- Title
Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL1038 from Burkholderia pseudomallei.
- Authors
Shaibullah, Sofiyah; Mohd-Sharif, Nurhikmah; Ho, Kok Lian; Firdaus-Raih, Mohd; Nathan, Sheila; Mohamed, Rahmah; Ng, Chyan Leong
- Abstract
Melioidosis is an infectious disease caused by the pathogenic bacterium Burkholderia pseudomallei. Whole-genome sequencing revealed that the B. pseudomallei genome includes 5855 coding DNA sequences (CDSs), of which ∼25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 Å resolution. It belonged to space group C2221, with unit-cell parameters a = 85.36, b = 115.63, c = 46.73 Å. The calculated Matthews coefficient ( VM) suggests that there are two molecules per asymmetric unit, with a solvent content of 48.8%.
- Subjects
CRYSTALLIZATION; SEPARATION (Technology); BURKHOLDERIA infections; BURKHOLDERIA; GRAM-negative bacteria
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2014, Vol 70, Issue 12, p1697
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X14025278