We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Dnali1 is required for sperm motility and male fertility in mice.
- Authors
Zhou, Yiling; Wang, Yaling; Chen, Jingwen; Wu, Bangguo; Tang, Shuyan; Zhang, Feng; Liu, Chunyu; Wang, Lingbo
- Abstract
Background: The sperm flagellum is an evolutionarily conserved specialized organelle responsible for sperm motility and male fertility. Deleterious mutations in genes involved in the sperm flagellum assembly can often cause sperm motility defects and male infertility. The murine Dnali1 gene encodes a protein that is known to interact with the cytoplasmic dynein heavy chain 1. Results: A Dnali1-mutated mouse model was generated by inducing a nonsense mutation in the Dnali1 gene. The Dnali1-mutated male mice presented impaired sperm motility and were completely infertile. Although no obviously abnormal sperm morphology was observed in Dnali1-mutated male mice, the ultrastructural structure of sperm flagellum was disrupted, displaying as an asymmetrical distribution of the longitudinal columns (LCs). Notably, infertile Dnali1-mutated male mice were able to obtain offspring via ICSI. Conclusions: Our results uncover a role of DNALI1 in sperm motility and male fertility in mice, and demonstrate that ICSI overcomes Dnali1-associated male infertility, thus providing guidance for the diagnosis and genetic counseling of DNALI1-associated human infertility.
- Subjects
MALE infertility; SPERM motility; FERTILITY; NONSENSE mutation; GENETIC counseling; MICE
- Publication
Basic & Clinical Andrology, 2023, Vol 33, Issue 1, p1
- ISSN
2051-4190
- Publication type
Article
- DOI
10.1186/s12610-023-00205-y