We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Galectin-4-Regulated Delivery of Glycoproteins to the Brush Border Membrane of Enterocyte-Like Cells.
- Authors
Stechly, Laurence; Morelle, Willy; Dessein, Anne-Frédérique; André, Sabine; Grard, Georges; Trinel, Dave; Dejonghe, Marie-José; Leteurtre, Emmanuelle; Drobecq, Hervé; Trugnan, Germain; Gabius, Hans Joachim; Huet, Guillemette
- Abstract
We have previously reported that silencing of galectin-4 expression in polarized HT-29 cells perturbed apical biosynthetic trafficking and resulted in a phenotype similar to the inhibitor of glycosylation, 1-benzyl-2-acetamido-2-deoxy-β-d-galactopyranoside (GalNAcα- O-bn). We now present evidence of a lipid raft-based galectin-4-dependent mechanism of apical delivery of glycoproteins in these cells. First, galectin-4 recruits the apical glycoproteins in detergent-resistant membranes (DRMs) because these glycoproteins were depleted in DRMs isolated from galectin-4-knockdown (KD) HT-29 5M12 cells. DRM-associated glycoproteins were identified as ligands for galectin-4. Structural analysis showed that DRMs were markedly enriched in a series of complex N-glycans in comparison to detergent-soluble membranes. Second, in galectin-4-KD cells, the apical glycoproteins still exit the Golgi but accumulated inside the cells, showing that their recruitment within lipid rafts and their apical trafficking required the delivery of galectin-4 at a post-Golgi level. This lectin that is synthesized on free cytoplasmic ribosomes is externalized from HT-29 cells mostly in the apical medium and follows an apical endocytic–recycling pathway that is required for the apical biosynthetic pathway. Together, our data show that the pattern of N-glycosylation of glycoproteins serves as a recognition signal for endocytosed galectin-4, which drives the raft-dependent apical pathway of glycoproteins in enterocyte-like HT-29 cells.
- Subjects
GLYCOPROTEINS; GLYCOCONJUGATES; GLYCOSYLATION; CELL membranes; CELLS; PROTEINS; LECTINS
- Publication
Traffic, 2009, Vol 10, Issue 4, p438
- ISSN
1398-9219
- Publication type
Article
- DOI
10.1111/j.1600-0854.2009.00882.x