We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cytotoxicity and Pro-Apoptotic, Antioxidant and Anti-Inflammatory Activities of Geopropolis Produced by the Stingless Bee Melipona fasciculata Smith.
- Authors
Barboza, Josianne Rocha; Pereira, Francisco Assis Nascimento; Fernandes, Renan Amphilophio; Vasconcelos, Cleydlenne Costa; Cartágenes, Maria do Socorro de Sousa; Oliveira Lopes, Alberto Jorge; Melo, Andreia Cristina de; Guimarães, Isabella dos Santos; Rocha, Cláudia Quintino da; Ribeiro, Maria Nilce de Sousa
- Abstract
Geopropolis is produced by some stingless bee species, such as Melipona fasciculata Smith, a native species from Brazil. This study aims to investigate the antioxidant and anti-inflammatory activities and cytotoxicity effects of geopropolis hydroethanolic extracts against lung (H460 and A549) and ovarian (A2780 and ES2) cancer cell lines and non-tumor (HUVEC) cell lines using chemical identification by LC/MS/MS analysis and in silico assays to determine which compounds are associated with bioactivity. The antioxidant activity of extracts and inhibitory activity against COX enzymes were assessed by in vitro assays; cytotoxicity effect was evaluated by the MTT assay; cell cycle was assessed by flow cytometry and apoptosis by Western blotting. The geopropolis extracts showed great radical scavenging potential, preferential inhibition of COX-2, decreased cancer cell viability, non-cytotoxic effects against the non-tumoral cell line, besides modulating the cell cycle and inducing cancer cell apoptosis through the activation of caspase-3 and PARP protein cleavage. The in silico study suggests that corilagin, typhaneoside, taraxerone and marsformosanone, identified by LC/MS/MS, can be associated with anti-inflammatory activity and cytotoxic effects. Thus, the current study suggests the potential of geopropolis concerning the research field of new pharmacological alternatives regarding cancer therapy.
- Subjects
BRAZIL; STINGLESS bees; ALTERNATIVE treatment for cancer; CANCER cells; CELL lines; POLY(ADP-ribose) polymerase; ANTINEOPLASTIC antibiotics
- Publication
Biology (2079-7737), 2020, Vol 9, Issue 9, p292
- ISSN
2079-7737
- Publication type
Article
- DOI
10.3390/biology9090292