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- Title
The clonal structure ofBacillus thuringiensisisolates from north-east Poland does not correlate with theircrygene diversity.
- Authors
Świ&ecedil;cicka, Izabela; Mahillon, Jacques
- Abstract
The genetic relationship among 103 naturalBacillus thuringiensisisolates was investigated on the basis of polymerase chain reaction amplification of their specific crystal (cry) protein type genes and chromosomal DNA profiling by pulsed-field gel electrophoresis (PFGE). The strains were recovered from the intestines of small wild rodents and insectivores from the Biebrza National Park and theŁomża Landscape Park of the Narew River Valley in north-east Poland. The percentage ofB. thuringiensisstrains harbouring genes coding for toxins active against Lepidoptera (cry1,cry2,cry9) was very high (64%) compared with that of Diptera-specific strains (cry4, 14%). No strain withcrygenes coding for proteins directed against coleopteran larvae and nematodes was found. After digestion withNotI andAscI, only nine PFGE pulsotypes were observed among all isolates, indicating a clonal structure for theB. thuringiensispopulation from NE Poland. Interestingly, no correlation was observed between the DNA pulsotype strains and their crystal gene content and diversity. These results therefore emphasize the importance ofcrygene horizontal transfer occurring among natural isolates ofB. thuringiensis.
- Subjects
GENETICS; CHROMOSOMES; DNA; NUCLEIC acids; ELECTROPHORESIS; DNA polymerases; POLYMERASE chain reaction
- Publication
Environmental Microbiology, 2005, Vol 7, Issue 1, p34
- ISSN
1462-2912
- Publication type
Article
- DOI
10.1111/j.1462-2920.2004.00662.x