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- Title
Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells.
- Authors
Amato, R; Scumaci, D; D'Antona, L; Iuliano, R; Menniti, M; Di Sanzo, M; Faniello, M C; Colao, E; Malatesta, P; Zingone, A; Agosti, V; Costanzo, F S; Mileo, A M; Paggi, M G; Lang, F; Cuda, G; Lavia, P; Perrotti, N
- Abstract
The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of epithelial sodium channel-mediated sodium transport and is involved in the transduction of growth factor-dependent cell survival and proliferation signals. Growing evidence now points to Sgk1 as a key element in the development and/or progression of human cancer. To gain insight into the mechanisms through which Sgk1 regulates cell proliferation, we adopted a proteomic approach to identify up- or downregulated proteins after Sgk1-specific RNA silencing. Among several proteins, the abundance of which was found to be up- or downregulated upon Sgk1 silencing, we focused our attention of RAN-binding protein 1 (RANBP1), a major effector of the GTPase RAN. We report that Sgk1-dependent regulation of RANBP1 has functional consequences on both mitotic microtubule activity and taxol sensitivity of cancer cells.
- Subjects
SGK protein; PACLITAXEL; CANCER cell proliferation; COLON cancer; EPITHELIAL cells; GENE silencing; GENETIC regulation
- Publication
Oncogene, 2013, Vol 32, Issue 38, p4572
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/onc.2012.470