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- Title
Detection and measurement of in vitro gene transfer by gamma camera imaging.
- Authors
Zinn, K R; Chaudhuri, T R; Buchsbaum, D J; Mountz, J M; Rogers, B E
- Abstract
The purpose of this work was to develop a high capacity method to image gene transfer to cancer cells growing as monolayers in cell culture plates. A sensitive and high capacity nuclear-imaging method for detection of gene transfer in vitro will allow rapid validation of vectors in different cell lines under various conditions. Human cancer cell lines (A-427 non-small cell lung, SKOV3.ip1 ovarian, MDAMB-468 breast, and BxPC-3 pancreatic) were infected with a replication-incompetent adenoviral vector encoding the human type 2 somatostatin receptor (Ad-hSSTr2). Expression of the hSSTr2 reporter protein in cells was detected by imaging an internalized [sup 99m]Tc-labeled, hSSTr2 binding peptide (P2045, Diatide, Inc.). Imaging provided an accurate measure of internally bound [sup 99m]Tc as evidenced by equivalence of results for imaging region of interest (ROI) analyses and gamma counter measurements. Internally bound [sup 99m]Tc-P2045 was linearly correlated (R² = 0.98) with the percentage of hSSTr2-positive cells following gene transfer. Excess P2045 blocked binding and internalization of the [sup 99m]Tc-P2045, indicating the specificity of the technique. Up to four 96-well plates could be imaged simultaneously, thereby demonstrating the high capacity of the system. This novel in vitro approach provides a new method to test enhanced gene transfer as new vectors are developed.
- Subjects
GENETIC transformation; CANCER cells
- Publication
Gene Therapy, 2001, Vol 8, Issue 4, p291
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3301391