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- Title
Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans.
- Authors
Le, Hoa Thi; Park, Kyu H.; Jung, Woong; Park, Hyung Soon; Kim, Tae Woo
- Abstract
We developed a new method for MALDI-TOF MS detection of N-glycans derived from human serum. The synergistic combination of microwave-assisted Girard T derivatization, solid-phase extraction desalting, and an ionic liquid matrix (2, 5-dihydroxybenzoic acid/aniline) (GT-SPE-DHB/An) allowed of more sensitive N-glycans detection than a conventional ionic liquid matrix in MALDI-TOF MS. The superior sensitivity of our method was confirmed by the number of assigned N-glycans in 900–2,000 m/z range. Using our GT-SPE-DHB/An method, we were successfully able to assign 31 glycans. However, with the established method, i.e., DHB/An method, only 15 glycans were assigned. To the best of our knowledge, this GT-SPE-DHB/An method is the first to combine cationic derivatization of N-glycan and ionic liquid matrix for N-glycan analysis in MALDI-TOF MS.
- Subjects
MICROWAVES; GLYCANS; IONIC liquids; ANILINE; SERUM
- Publication
Journal of Analytical Methods in Chemistry, 2018, p1
- ISSN
2090-8865
- Publication type
Article
- DOI
10.1155/2018/7832987