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- Title
Deregulated expression of fat and muscle genes in B-cell chronic lymphocytic leukemia with high lipoprotein lipase expression.
- Authors
Bilban, M.; Heintel, D.; Scharl, T.; Woelfel, T.; Auer, M. M.; Porpaczy, E.; Kainz, B.; Kröber, A.; Carey, V. J.; Shehata, M.; Zielinski, C.; Pickl, W.; Stilgenbauer, S.; Gaiger, A.; Wagner, O.; Jäger, U.; Kröber, A; Jäger, U; German CLL Study Group
- Abstract
Lipoprotein lipase (LPL) is a prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL) related to immunoglobulin V(H) gene (IgV(H))mutational status. We determined gene expression profiles using Affymetrix U133A GeneChips in two groups of B-CLLs selected for either high ('LPL+', n=10) or low ('LPL-', n=10) LPL mRNA expression. Selected genes were verified by real-time PCR in an extended patient cohort (n=42). A total of 111 genes discriminated LPL+ from LPL- B-CLLs. Of these, the top three genes associated with time to first treatment were Septin10, DMD and Gravin (P</=0.01). The relationship of LPL+ and LPL- B-CLL gene expression signatures to 52 tissues was statistically analyzed. The LPL+ B-CLL expression signature, represented by 64 genes was significantly related to fat, muscle and PB dendritic cells (P<0.001). Exploration of microarray data to define functional alterations related to the biology of LPL+ CLL identified two functional modules, fatty acid degradation and MTA3 signaling, as being altered with higher statistical significance. Our data show that LPL+ B-CLL cells have not only acquired gene expression changes in fat and muscle-associated genes but also in functional pathways related to fatty acid degradation and signaling which may ultimately influence CLL biology and clinical outcome.
- Subjects
CHRONIC lymphocytic leukemia; LIPOPROTEIN lipase; GENE expression; DYSTROPHIN; DENDRITIC cells; ANTIGEN presenting cells
- Publication
Leukemia (08876924), 2006, Vol 20, Issue 6, p1080
- ISSN
0887-6924
- Publication type
journal article
- DOI
10.1038/sj.leu.2404220