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- Title
Radiation-enhanced expression of interferon-inducible genes in the KG1a primitive hematopoietic cell line.
- Authors
Clave, E; Carosella, E D; Gluckman, E; Socié, G
- Abstract
X-ray treatment induces a complex molecular response in hematopoietic cells leading to cell death. Using the mRNA differential display technique, we searched for genes whose expression was modified by ionizing radiation (IR) in the human p53-deficient leukemic cell line KG1a. We isolated a partial cDNA corresponding to the interferon (IFN)-inducible 1-8d gene. The expression of both 1-8d and 9-27, another gene from the same IFN-inducible family, was increased 24 and 48 h following irradiation. We did not find enhancement of either IFNgamma mRNA or interferon regulatory factor-1 (IRF-1) mRNA in irradiated KG1a cells, indicating that 1-8d and 9-27 enhancement was not due to an IFN activation. Thus, the induction of IFN-inducible genes by IR may provide a link between radiation-induced and IFN-mediated cell death.
- Subjects
IONIZING radiation; CELL lines; GENE expression; RNA metabolism; CELL death; DNA; DOCUMENTATION; GENE amplification; GENES; INTERFERONS; NUCLEOTIDES; PHOSPHOPROTEINS; PROTEINS; RNA; DNA-binding proteins; SEQUENCE analysis; PHYSIOLOGICAL effects of radiation
- Publication
Leukemia (08876924), 1997, Vol 11, Issue 1, p114
- ISSN
0887-6924
- Publication type
journal article
- DOI
10.1038/sj.leu.2400528