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- Title
QTL Analysis of Soybean Seed Coat Discoloration Associated with II TT Genotype.
- Authors
Oyoo, Maurice E.; Benitez, Eduardo R.; Kurosaki, Hideki; Ohnishi, Shizen; Miyoshi, Tomoaki; Kiribuchi-Otobe, Chikako; Horigane, Akira; Takahashi, Ryoji
- Abstract
In soybean [Glycine max (L.) Merr.], the domi- nant allele of the T locus for tawny pubescence is associated with chilling tolerance and is useful for breeding for chilling tolerance. Yellow hilum cultivars with the dominant I allele are preferred in Japan because of a better external appear- ance. However, the II TT allelic combination darkens the entire seed coat and degrades the external appearance. This study was conducted to investigate the genetic basis of seed coat dis- coloration using DNA markers. F2 populations derived from crosses between soybean breed- ing lines 0518BW-8 with slight discoloration and 0734BW-1 with severe discoloration were grown at Memuro in 2003 and Kunneppu in 2007. Seed coat discoloration was measured by the L* value with a two-dimensional colorimeter. A total of 104 simple sequence repeat markers were clas- sified into 20 linkage groups (LGs) spanning 1025 cM in 2003 and 19 LGs spanning 789 cM in 2007. A quantitative trait locus (QTL), discoll, was identified by composite interval mapping in LG A2 (chromosome 8) across years and loca- tions. It had an LOD score of 5.58 explaining 22.0% of phenotypic variance in 2003 and a LOD score of 5.62 explaining 20.2% of variance in 2007. The allele from 0518BW-8 increased the L* value at the QTL. Position of discoll was close to the I locus corresponding to the chal- cone synthase (CHS) multigene family clus- ter. No sequence differences were observed between the two lines in nucleotide sequences of CHS1 and CHS3 genes and the intergenic region between CHS1 and CHS3.
- Subjects
SOYBEAN; EFFECT of cold on plants; LOCUS (Genetics); GENETIC polymorphisms; NUCLEOTIDE sequence; LINKAGE (Genetics)
- Publication
Crop Science, 2011, Vol 51, Issue 2, p464
- ISSN
0011-183X
- Publication type
Article
- DOI
10.2135/cropsci2010.02.0121