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- Title
STUDIES ON THE PRODUCTION OF ENZYMIC PREPARATIONS FROM BIOTECHNOLOGICALLY IMPORTANT MICROMYCETES.
- Authors
Carpa, Rahela; Drăgan-Bularda, Mihail
- Abstract
Three species of micromycetes, Fusarium oxysporum, Penicillium citrinum and Botrytis paeoniae, were studied for production of the following enzymes: amylase, dextranase, levanase and inulinase. A mineral medium was used as a nutritive base, to which was added 2% or 0.2% of specific substrate (starch, dextran, levan and inulin). These served to induce the synthesis of the enzymes. Enzymic activities were determined qualitatively by paper chromatography, and quantitatively by determining reducing sugars. It was established that most of the enzymes were secreted into the culture medium. Hydrolysis values of over 50% were recorded. A smaller quantity of each enzyme remained bound to the mycelium, and due to this, hydrolysis values were lower. As regards the production of the five enzymes, the most active species was Fusarium oxysporum, then Penicillium citrinum. Several organic substances were used as growth stimulators and for the synthesis of the enzymes: yeast extract, maltose, sucrose and glucose. Yeast extract stimulated the production of all these enzymes in all three species; maltose stimulated the production of amylase, while glucose and sucrose stimulated the production of inulinase.
- Subjects
MICROFUNGI; ENZYMES; AMYLASES; DEXTRANASE; FUSARIUM oxysporum; MYCELIUM; PENICILLIUM; BOTRYTIS
- Publication
Contributii Botanice, 2010, Vol 45, p71
- ISSN
0069-9616
- Publication type
Article