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- Title
Superantigen-induced peripheral T-cell deletion: the effects of chemical modification of antigen-presenting cells, interleukin-4 and glucocorticoid hormones.
- Authors
Ayroldi, B.; Cannarile, L.; D'Adamio, F.; Riccardi, C.
- Abstract
Experiments were performed to evaluate the role of antigen-presenting cells (APC) and the effect of interleukin-4 (IL-4) and glucocorticoid hormone (CCH) exposure on the in vitro deletion of CD4+ CD8- and CD8+ CD4- T cells by staphylococcal enterotoxin B (SEB). APC fixation with the chemical cross-linker 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (ECDI)inhibited their capacity to induce SEB-specific deletion of mature T lymphocytes. Deletion was not influenced by treatment with anti-CD28 antibodies, which modulate T-cell activation. However, it was augmented by IL-4, known to counteract anti-CD3- and GCH-induced thymocyte apoptosis, and was inhibited by dexamethasone (DEX). These results indicate that metabolically active APC are required for deletion of antigen-specific mature T cells and suggest that IL-4 and GCH can modulate this phenomenon in vitro.
- Subjects
ANTIGEN presenting cells; IMMUNOCOMPETENT cells; INTERLEUKIN-4; GLUCOCORTICOIDS; T cells; THYMUS
- Publication
Immunology, 1995, Vol 84, Issue 4, p528
- ISSN
0019-2805
- Publication type
Article