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- Title
Recombinational DNA double-strand breaks in mice precede synapsis.
- Authors
Mahadevaiah, Shantha K.; Turner, James M.A.; Baudat, Frédéric; Rogakou, Emmy P.; de Boer, Peter; Blanco-Rodríguez, Josefa; Jasin, Maria; Keeney, Scott; Bonner, William M.; Burgoyne, Paul S.
- Abstract
In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (γ-H2AX, which marks the sites of DSBs) to investigate the timing, distribution and Spo11-dependence of meiotic DSBs in the mouse. We show that, as in yeast, recombination in the mouse is initiated by Spo11-dependent DSBs that form during leptotene. Loss of γ-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'.
- Subjects
GENETIC recombination; MICE; ANIMAL genetics
- Publication
Nature Genetics, 2001, Vol 27, Issue 3, p271
- ISSN
1061-4036
- Publication type
Article
- DOI
10.1038/85830