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- Title
Different pathways of human T-cell activation revealed by PHA-P and PHA-M.
- Authors
O'Flynn, K.; Russul-Saib, M.; Ando, I.; Wallace, Diana L.; Beverley, P. C. L.; Boylstont, A. W.; Linch, D. C.
- Abstract
Antigen-specific T-cell activation is mediated via the CD3-T1(antigen receptor) complex. and monoclonal antibodies to both CD3 and T1 cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other I cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA- M) induces calcium moblization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M arc thus mediated predominantly through different activation pathways.
- Subjects
T cells; MONOCLONAL antibodies; IMMUNOGLOBULINS; CELL culture; EPITOPES; LYMPHOCYTES
- Publication
Immunology, 1986, Vol 57, Issue 1, p55
- ISSN
0019-2805
- Publication type
Article