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- Title
New Transcriptional Reporters to Quantify and Monitor PPARγ Activity.
- Authors
Degrelle, Séverine A.; Shoaito, Hussein; Fournier, Thierry
- Abstract
The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPARγ activity in different cell types and screen for PPARγ’s potential ligands.
- Subjects
PEROXISOME proliferator-activated receptors; ADIPOGENESIS; LIPID metabolism; HISTONES; LUCIFERASES
- Publication
PPAR Research, 2017, p1
- ISSN
1687-4757
- Publication type
Article
- DOI
10.1155/2017/6139107